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human normal liver cell line l02  (iCell Bioscience Inc)

 
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    iCell Bioscience Inc human normal liver cell line l02
    Human Normal Liver Cell Line L02, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+liver+cell+line+l02/l02+cells/pm40543694-89-13-22
    Average 90 stars, based on 1 article reviews
    human normal liver cell line l02 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: PPM-18, an Analog of Vitamin K, Induces Autophagy and Apoptosis in Bladder Cancer Cells Through ROS and AMPK Signaling Pathways
    Article Snippet: Human normal liver cell line L02 was purchased from iCell Bioscience Inc. (Shanghai, China).

    Article Title: Mechanism of action of Pulsatilla chinensis (Bunge) Regel compounds in hepatocellular carcinoma (HCC) treatment: An integrated analysis combining network pharmacology, molecular docking, molecular dynamics simulations and luciferase reporter gene assay.
    Article Snippet: Ethnopharmacological relevance: Hepatocellular carcinoma (HCC) is a primary malignancy originating from hepatocytes in the liver parenchyma.. Pulsatilla chinensis (Bunge) Regel(P. chinensis) (verified via http://www.th eplantlist.org, accessed April 5, 2025), a perennial herb of the Ranunculaceae family, contains multiple bioactive constituents with demonstrated pharmacological effects, including antitumor, anti-inflammatory, antibacterial, antiviral, and immunomodulatory activities.. Aim of the study: To investigate the mechanisms of action and pharmacodynamic material basis of active compounds from P. chinensis against HCC cells.



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    Gq-DNA transfection effectively target tumor cells with high-level nucleolin. ( A ) Relative mRNA expression of nucleolin (NCL) mRNA in HepG2 and <t>L02.</t> ** p < 0.01. ( B ) Relative luciferase activities (Rel. Lucif. Act.) in HepG2 and L02 treated with polybrene (PB, 5 μg/mL) and CMV-Luc or Gq-CMV-Luc. ** p < 0.01. ( C ) Cellular uptake of DNA and Gq-DNA by HepG2 cells and L02. Cells were treated with polybrene (PB, 5 μg/mL) and YOYO-1-stained DNA and Gq-DNA for 6 h, respectively. Cellular uptake efficiency was tested by fluorescence microscope. Scale bar, 50 μm.
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    Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

    doi: 10.2147/JHC.S500119

    Figure Lengend Snippet: Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

    Article Snippet: The human normal liver cell line L02 was obtained from Beijing Baiao Bowei Biotechnology Co., Ltd.

    Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR

    High glucose upregulated the expression of NCAPD2. A. The relative mRNA expression of NCAPD2 in different HCC cells and normal liver cell L02. B. The protein expression of NCAPD2 in different HCC cells and normal liver cell L02. C. CCK-8 assay detected the cell viability of HCC cells treated with different concentration of glucose. D, E. The mRNA and protein expression of NCAPD2 in HCC cells treated with high glucose. ***P < 0.001.

    Journal: American Journal of Cancer Research

    Article Title: High glucose-induced NCAPD2 upregulation promotes malignant phenotypes and regulates EMT via the Wnt/β-catenin signaling pathway in HCC

    doi: 10.62347/HYNZ9211

    Figure Lengend Snippet: High glucose upregulated the expression of NCAPD2. A. The relative mRNA expression of NCAPD2 in different HCC cells and normal liver cell L02. B. The protein expression of NCAPD2 in different HCC cells and normal liver cell L02. C. CCK-8 assay detected the cell viability of HCC cells treated with different concentration of glucose. D, E. The mRNA and protein expression of NCAPD2 in HCC cells treated with high glucose. ***P < 0.001.

    Article Snippet: HCC cells (Huh7, SNU449, HepG2, and MHCC97H) and the human normal liver cell line L02 were obtained from Procell Life Science & Technology (Wuhan, China).

    Techniques: Expressing, CCK-8 Assay, Concentration Assay

    Linc-KILH is up-regulated in HCC tissues and correlated with the prognosis of patients with HCC. ( A) The expression level of Linc-KILH was detected by RT-qPCR in HCC tumor tissues and adjacent non-tumor liver tissues. (B) Relative expression of Linc-KILH in human L02 hepatocytes and HCC cell lines was detected by RT-qPCR. (C) The subcellular location of Linc-KILH was analyzed through fluorescence in situ hybridization (FISH) (original magnification ×400). (D) The overall survival and recurrence-free survival rates of 116 HCC patients were compared between higher Linc-KILH and lower Linc-KILH groups. *** P < 0.001.

    Journal: International Journal of Biological Sciences

    Article Title: Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma

    doi: 10.7150/ijbs.52279

    Figure Lengend Snippet: Linc-KILH is up-regulated in HCC tissues and correlated with the prognosis of patients with HCC. ( A) The expression level of Linc-KILH was detected by RT-qPCR in HCC tumor tissues and adjacent non-tumor liver tissues. (B) Relative expression of Linc-KILH in human L02 hepatocytes and HCC cell lines was detected by RT-qPCR. (C) The subcellular location of Linc-KILH was analyzed through fluorescence in situ hybridization (FISH) (original magnification ×400). (D) The overall survival and recurrence-free survival rates of 116 HCC patients were compared between higher Linc-KILH and lower Linc-KILH groups. *** P < 0.001.

    Article Snippet: Human HCC cell lines (SMMC-7721, Huh7, HepG2, MHCC-97H, MHCC-97L, Hep3B) and the human normal liver L02 cell line were obtained from KeyGen (Nanjing KeyGen Biotech Co., Ltd., Jiangsu, China).

    Techniques: Expressing, Quantitative RT-PCR, Fluorescence, In Situ Hybridization

    Gq-DNA transfection effectively target tumor cells with high-level nucleolin. ( A ) Relative mRNA expression of nucleolin (NCL) mRNA in HepG2 and L02. ** p < 0.01. ( B ) Relative luciferase activities (Rel. Lucif. Act.) in HepG2 and L02 treated with polybrene (PB, 5 μg/mL) and CMV-Luc or Gq-CMV-Luc. ** p < 0.01. ( C ) Cellular uptake of DNA and Gq-DNA by HepG2 cells and L02. Cells were treated with polybrene (PB, 5 μg/mL) and YOYO-1-stained DNA and Gq-DNA for 6 h, respectively. Cellular uptake efficiency was tested by fluorescence microscope. Scale bar, 50 μm.

    Journal: Pharmaceutics

    Article Title: G-Quadruplex Linked DNA Guides Selective Transfection into Nucleolin-Overexpressing Cancer Cells

    doi: 10.3390/pharmaceutics14102247

    Figure Lengend Snippet: Gq-DNA transfection effectively target tumor cells with high-level nucleolin. ( A ) Relative mRNA expression of nucleolin (NCL) mRNA in HepG2 and L02. ** p < 0.01. ( B ) Relative luciferase activities (Rel. Lucif. Act.) in HepG2 and L02 treated with polybrene (PB, 5 μg/mL) and CMV-Luc or Gq-CMV-Luc. ** p < 0.01. ( C ) Cellular uptake of DNA and Gq-DNA by HepG2 cells and L02. Cells were treated with polybrene (PB, 5 μg/mL) and YOYO-1-stained DNA and Gq-DNA for 6 h, respectively. Cellular uptake efficiency was tested by fluorescence microscope. Scale bar, 50 μm.

    Article Snippet: Human normal liver cell line L02 was obtained from CCTCC.

    Techniques: Transfection, Expressing, Luciferase, Staining, Fluorescence, Microscopy